Anti-Neisseria meningitidis NHBA/Dextranase Recombinant Antibody (5H2) (JN891033)

Name: Anti-Neisseria meningitidis NHBA/Dextranase Recombinant Antibody (5H2)
Brand: abinScience
SKU: JN891033
Price: 126 USD
Availability: InStock

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Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

JN891033

Reactivité des espèces

Neisseria meningitidis

Applications

ELISA, SPR

Espèce hôte

Human

Clonalité

Monoclonal

Isotype

IgG1, kappa

ID de clone

5H2

Cible

nhba, gna2132, Heparin binding protein, Neisserial heparin binding antigen, Genome-derived neisserial antigen 2132, Putative lipoprotein GNA2132, NHBA

Niveau d'endotoxines

Please contact with the lab for this information.

Pureté

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Numéro d'accès

Q9JPP1

RRID

Anti-Neisseria meningitidis NHBA/Dextranase Antibody (5H2) (abinScience Cat# JN891033, RRID:AB_3727794)
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Forme

Liquid

Tampon de stockage

0.01M PBS pH 7.4

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:2000-1:20000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Contexte

Neisserial heparin binding antigen (NHBA/Dextranase) is a ~44 kDa protein. A major human immunogenic protein detected in patients recovering from meningitidis, where it induces bactericidal antibodies. Binds human cells, heparin and heparan sulfate proteoglycan in vitro via the Arg-rich motif. Heparin-binding to this protein protects bacteria against killing by bactericidal antibodies (serum killing). The bacteria binds a number of human extracellular sialyated and/or sulfated glycans via this protein, including chondroitin sulfate, heparin and ganglioside GT3. Binds DNA non-specifically.

1. Maritan, M. et al. (2018) PloS one 13, e0201922. PMID: 30133484

Note

For research use only.

Images

Bioactivity

Detects Neisseria meningitidis NHBA/Dextranase in indirect ELISAs.
SDS-PAGE

SDS-PAGE for Anti-Neisseria meningitidis NHBA/Dextranase Antibody (5H2).
Western blot

Western blot analysis was performed using anti-Neisseria meningitidis NHBA/Dextranase monoclonal antibody at 1µg/ml on various samples.
Lane 1: recombinant Neisseria meningitidis NHBA (Catalog No: JN891012)
Lane 2: negative control

Reférences

Fiche technique

Anti-Neisseria meningitidis NHBA/Dextranase Recombinant Antibody (5H2).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

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