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| カタログ番号 | HV187014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-NFKBID Polyclonal Antibody (HV187014) is a rabbit polyclonal antibody detecting NFKBID in ELISA, IHC, WB. Suitable for Human and Mouse.
Highlights
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| 種反応性 | Human | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human NFKBID (Asp46-Val285). | ||||||||
| ターゲット | NFKB inhibitor delta, IkB-delta, TA-NFKBH, NF-kappa-B inhibitor delta, IkappaBNS, I-kappa-B-delta, T-cell activation NFKB-like protein, IKBNS, IkappaBdelta, NFKBID | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | Q8NI38 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | NF-kappa-B inhibitor delta (NFKBID) is a ~33 kDa protein. Regulates the expression of IL-2, IL-6, and other cytokines through regulation on NF-kappa-B activity. Functions in the regulation of inflammatory responses. Involved in the induction of T helper 17 cells (Th17) differentiation upon recognition of antigen by T cell antigen receptor (TCR). May also regulate TCR-induced negative selection of thymocytes. | ||||||||
| 注意事項 | For research use only. |
Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting NFKBID (HV187014) at a concentration of 12.6 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of NFKBID was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
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