Anti-PARP1 Polyclonal Antibody (HY482014)

Name: Anti-PARP1 Polyclonal Antibody
Brand: abinScience
SKU: HY482014
Price: 96 USD
Availability: InStock

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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号

HY482014

説明

Anti-PARP1 Polyclonal Antibody (HY482014) is a rabbit polyclonal antibody detecting PARP1 in ELISA, IHC, WB. Suitable for Mouse, Bovine, Human, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

種反応性

Human

アプリケーション

ELISA, IHC, WB

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

免疫原

E. coli - derived recombinant Human PARP1 (Thr373-Glu540).

ターゲット

PARP-1, Protein poly-ADP-ribosyltransferase PARP1, DNA ADP-ribosyltransferase PARP1, Poly [ADP-ribose] polymerase 1, PPOL, Poly[ADP-ribose] synthase 1, ADPRT 1, ADP-ribosyltransferase diphtheria toxin-like 1, ADPRT, PARP1, NAD(+) ADP-ribosyltransferase 1, ARTD1

精製

Purified by antigen affinity column.

アクセッション番号

P09874

形態

Liquid

保存バッファー

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報

アプリケーション	希釈
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

安定性と保存

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Poly [ADP-ribose] polymerase 1 (PARP1) is a ~113 kDa protein. Poly-ADP-ribosyltransferase that mediates poly-ADP-ribosylation of proteins and plays a key role in DNA repair. Mediates glutamate, aspartate, serine, histidine or tyrosine ADP-ribosylation of proteins: the ADP-D-ribosyl group of NAD(+) is transferred to the acceptor carboxyl group of target residues and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units. Serine ADP-ribosylation of proteins constitutes the primary form of ADP-ribosylation of proteins in response to DNA damage. Specificity for the different amino acids is conferred by interacting factors, such as HPF1 and NMNAT1. Following interaction with HPF1, catalyzes serine ADP-ribosylation of target proteins; HPF1 confers serine specificity by completing the PARP1 active site.

1. Maruyama, T. et al. (2007) Clinical and experimental immunology 147, 164-75. PMID: 17177976

2. Langelier, MF. et al. (2008) The Journal of biological chemistry 283, 4105-14. PMID: 18055453

3. Ahel, I. et al. (2008) Nature 451, 81-5. PMID: 18172500

4. Reinemund, J. et al. (2009) Biochemical pharmacology 77, 1795-805. PMID: 19344625

5. Ahel, D. et al. (2009) Science (New York, N.Y.) 325, 1240-3. PMID: 19661379

6. Langelier, MF. et al. (2010) The Journal of biological chemistry 285, 18877-87. PMID: 20388712

7. Mao, Z. et al. (2011) Science (New York, N.Y.) 332, 1443-6. PMID: 21680843

8. Langelier, MF. et al. (2012) Science (New York, N.Y.) 336, 728-32. PMID: 22582261

9. Yan, Q. et al. (2013) Molecular and cellular biology 33, 845-57. PMID: 23230272

10. Vyas, S. et al. (2014) Nature communications 5, 4426. PMID: 25043379

注意事項

For research use only.

イメージ

Western blot

Western blot analysis was performed using anti-PARP1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: Jurkat cell lysate
Lane 2: Hela cell lysate
Lane 3: HepG2 cell lysate
Lane 4: 293T cell lysate
Lane 5: A549 cell lysate
Lane 6: K562 cell lysate