ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
20.13 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Poly [ADP-ribose] polymerase 1 (PARP1) is a ~113 kDa protein. Poly-ADP-ribosyltransferase that mediates poly-ADP-ribosylation of proteins and plays a key role in DNA repair. Mediates glutamate, aspartate, serine, histidine or tyrosine ADP-ribosylation of proteins: the ADP-D-ribosyl group of NAD(+) is transferred to the acceptor carboxyl group of target residues and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units. Serine ADP-ribosylation of proteins constitutes the primary form of ADP-ribosylation of proteins in response to DNA damage. Specificity for the different amino acids is conferred by interacting factors, such as HPF1 and NMNAT1. Following interaction with HPF1, catalyzes serine ADP-ribosylation of target proteins; HPF1 confers serine specificity by completing the PARP1 active site.
1. Maruyama, T. et al. (2007) Clinical and experimental immunology 147, 164-75. PMID: 17177976
2. Langelier, MF. et al. (2008) The Journal of biological chemistry 283, 4105-14. PMID: 18055453
3. Ahel, I. et al. (2008) Nature 451, 81-5. PMID: 18172500
4. Reinemund, J. et al. (2009) Biochemical pharmacology 77, 1795-805. PMID: 19344625
5. Ahel, D. et al. (2009) Science (New York, N.Y.) 325, 1240-3. PMID: 19661379
6. Langelier, MF. et al. (2010) The Journal of biological chemistry 285, 18877-87. PMID: 20388712
7. Mao, Z. et al. (2011) Science (New York, N.Y.) 332, 1443-6. PMID: 21680843
8. Langelier, MF. et al. (2012) Science (New York, N.Y.) 336, 728-32. PMID: 22582261
9. Yan, Q. et al. (2013) Molecular and cellular biology 33, 845-57. PMID: 23230272
10. Vyas, S. et al. (2014) Nature communications 5, 4426. PMID: 25043379
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Human PARP1 Protein, N-His
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