Anti-PARP1 Polyclonal Antibody (HY482014) is a rabbit polyclonal antibody detecting PARP1 in ELISA, IHC, WB. Suitable for Mouse, Bovine, Human, and Rat.
Highlights
●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.
Species reactivity
Mouse, Bos taurus, Human, Rat
Applications
ELISA, IHC, WB
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human PARP1 (Thr373-Glu540).
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application
Dilution
ELISA
1:5000-1:20000
IHC
1:50-1:500
WB
1:500-1:2000
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background
Poly [ADP-ribose] polymerase 1 (PARP1) is a ~113 kDa protein. Poly-ADP-ribosyltransferase that mediates poly-ADP-ribosylation of proteins and plays a key role in DNA repair. Mediates glutamate, aspartate, serine, histidine or tyrosine ADP-ribosylation of proteins: the ADP-D-ribosyl group of NAD(+) is transferred to the acceptor carboxyl group of target residues and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units. Serine ADP-ribosylation of proteins constitutes the primary form of ADP-ribosylation of proteins in response to DNA damage. Specificity for the different amino acids is conferred by interacting factors, such as HPF1 and NMNAT1. Following interaction with HPF1, catalyzes serine ADP-ribosylation of target proteins; HPF1 confers serine specificity by completing the PARP1 active site.
1. Maruyama, T. et al. (2007) Clinical and experimental immunology 147, 164-75. PMID: 17177976
2. Langelier, MF. et al. (2008) The Journal of biological chemistry 283, 4105-14. PMID: 18055453
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6. Langelier, MF. et al. (2010) The Journal of biological chemistry 285, 18877-87. PMID: 20388712
7. Mao, Z. et al. (2011) Science (New York, N.Y.) 332, 1443-6. PMID: 21680843
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9. Yan, Q. et al. (2013) Molecular and cellular biology 33, 845-57. PMID: 23230272
10. Vyas, S. et al. (2014) Nature communications 5, 4426. PMID: 25043379
Note
For research use only.
Images
Western blot
Western blot analysis was performed using anti-PARP1 polyclonal antibody at 1μg/ml on various samples. Lane 1: Jurkat cell lysate Lane 2: Hela cell lysate Lane 3: HepG2 cell lysate Lane 4: 293T cell lysate Lane 5: A549 cell lysate Lane 6: K562 cell lysate
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