Anti-PARP1 Polyclonal Antibody (HY482014)

Name: Anti-PARP1 Polyclonal Antibody
Brand: abinScience
SKU: HY482014
Price: 96 USD
Availability: InStock

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Обзор

Каталожный номер

HY482014

Описание

Anti-PARP1 Polyclonal Antibody (HY482014) is a rabbit polyclonal antibody detecting PARP1 in ELISA, IHC, WB. Suitable for Mouse, Bovine, Human, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Реактивность видов

Human

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Human PARP1 (Thr373-Glu540).

Цель

PARP-1, Protein poly-ADP-ribosyltransferase PARP1, DNA ADP-ribosyltransferase PARP1, Poly [ADP-ribose] polymerase 1, PPOL, Poly[ADP-ribose] synthase 1, ADPRT 1, ADP-ribosyltransferase diphtheria toxin-like 1, ADPRT, PARP1, NAD(+) ADP-ribosyltransferase 1, ARTD1

Очистка

Purified by antigen affinity column.

Номер доступа

P09874

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

Poly [ADP-ribose] polymerase 1 (PARP1) is a ~113 kDa protein. Poly-ADP-ribosyltransferase that mediates poly-ADP-ribosylation of proteins and plays a key role in DNA repair. Mediates glutamate, aspartate, serine, histidine or tyrosine ADP-ribosylation of proteins: the ADP-D-ribosyl group of NAD(+) is transferred to the acceptor carboxyl group of target residues and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units. Serine ADP-ribosylation of proteins constitutes the primary form of ADP-ribosylation of proteins in response to DNA damage. Specificity for the different amino acids is conferred by interacting factors, such as HPF1 and NMNAT1. Following interaction with HPF1, catalyzes serine ADP-ribosylation of target proteins; HPF1 confers serine specificity by completing the PARP1 active site.

1. Maruyama, T. et al. (2007) Clinical and experimental immunology 147, 164-75. PMID: 17177976

2. Langelier, MF. et al. (2008) The Journal of biological chemistry 283, 4105-14. PMID: 18055453

3. Ahel, I. et al. (2008) Nature 451, 81-5. PMID: 18172500

4. Reinemund, J. et al. (2009) Biochemical pharmacology 77, 1795-805. PMID: 19344625

5. Ahel, D. et al. (2009) Science (New York, N.Y.) 325, 1240-3. PMID: 19661379

6. Langelier, MF. et al. (2010) The Journal of biological chemistry 285, 18877-87. PMID: 20388712

7. Mao, Z. et al. (2011) Science (New York, N.Y.) 332, 1443-6. PMID: 21680843

8. Langelier, MF. et al. (2012) Science (New York, N.Y.) 336, 728-32. PMID: 22582261

9. Yan, Q. et al. (2013) Molecular and cellular biology 33, 845-57. PMID: 23230272

10. Vyas, S. et al. (2014) Nature communications 5, 4426. PMID: 25043379

Примечание

For research use only.

Изображения

Western blot

Western blot analysis was performed using anti-PARP1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: Jurkat cell lysate
Lane 2: Hela cell lysate
Lane 3: HepG2 cell lysate
Lane 4: 293T cell lysate
Lane 5: A549 cell lysate
Lane 6: K562 cell lysate