Anti-PTN Polyclonal Antibody (HB853014) is a rabbit polyclonal antibody detecting PTN in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Chicken.
Highlights
●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.
Реактивность видов
Human, Mouse, Rat
Применения
ELISA, IHC, WB
Хозяинский вид
Rabbit
Клоональность
Polyclonal
Изотип
IgG
Иммуноген
E. coli - derived recombinant Human PTN (Gly33-Asp168).
Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Информация об использовании продукта
Применение
Разбавление
ELISA
1:5000-1:20000
IHC
1:50-1:500
WB
1:500-1:2000
Устойчивость и хранение
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон
Pleiotrophin (PTN) is a ~18 kDa protein. Secreted growth factor that mediates its signal through cell-surface proteoglycan and non-proteoglycan receptors. Binds cell-surface proteoglycan receptor via their chondroitin sulfate (CS) groups. Thereby regulates many processes like cell proliferation, cell survival, cell growth, cell differentiation and cell migration in several tissues namely neuron and bone. Also plays a role in synaptic plasticity and learning-related behavior by inhibiting long-term synaptic potentiation. Binds PTPRZ1, leading to neutralization of the negative charges of the CS chains of PTPRZ1, inducing PTPRZ1 clustering, thereby causing the dimerization and inactivation of its phosphatase activity leading to increased tyrosine phosphorylation of each of the PTPRZ1 substrates like ALK, CTNNB1 or AFAP1L2 in order to activate the PI3K-AKT pathway.
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