Anti-Human PTN Recombinant Antibody (SAA1600) (HB853013)

Name: Anti-Human PTN Recombinant Antibody (SAA1600)
Brand: abinScience
SKU: HB853013
Price: 196 USD
Availability: InStock

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50ug
100ug
1mg

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HB853013

Species reactivity

Human

Applications

ELISA

Host species

Mouse

Isotype

IgG2a, kappa

Clone ID

SAA1600

Clonality

Monoclonal

Target

Heparin-binding neurite outgrowth-promoting factor 1, Heparin-binding brain mitogen, PTN, HBGF-8, Pleiotrophin, Heparin-binding growth factor 8, HBBM, HBNF-1, NEGF1, Osteoblast-specific factor 1, HBNF, HBNF1, Heparin-binding growth-associated molecule, HB-GAM, OSF-1, Heparin-binding neurite outgrowth-promoting factor

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P21246

RRID

Anti-Human PTN Antibody (SAA1600) (abinScience Cat# HB853013, RRID:AB_3727361)

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Pleiotrophin (PTN) is a ~18 kDa protein. Secreted growth factor that mediates its signal through cell-surface proteoglycan and non-proteoglycan receptors. Binds cell-surface proteoglycan receptor via their chondroitin sulfate (CS) groups. Thereby regulates many processes like cell proliferation, cell survival, cell growth, cell differentiation and cell migration in several tissues namely neuron and bone. Also plays a role in synaptic plasticity and learning-related behavior by inhibiting long-term synaptic potentiation. Binds PTPRZ1, leading to neutralization of the negative charges of the CS chains of PTPRZ1, inducing PTPRZ1 clustering, thereby causing the dimerization and inactivation of its phosphatase activity leading to increased tyrosine phosphorylation of each of the PTPRZ1 substrates like ALK, CTNNB1 or AFAP1L2 in order to activate the PI3K-AKT pathway.

1. Stoica, GE. et al. (2001) The Journal of biological chemistry 276, 16772-9. PMID: 11278720

2. Fukada, M. et al. (2006) FEBS letters 580, 4051-6. PMID: 16814777

3. Mikelis, C. et al. (2009) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 23, 1459-69. PMID: 19141530

4. Ryan, E. et al. (2016) The FEBS journal 283, 1488-503. PMID: 26896299

5. Kuboyama, K. et al. (2016) The Journal of biological chemistry 291, 18117-28. PMID: 27445335

6. Wellstein, A. et al. (1992) The Journal of biological chemistry 267, 2582-7. PMID: 1733956

7. Kretschmer, PJ. et al. (1991) Growth factors (Chur, Switzerland) 5, 99-114. PMID: 1768439

8. Imai, S. et al. (2009) Bone 44, 785-94. PMID: 19442624

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Human PTN Antibody (SAA1600)

References

Datasheet

Anti-Human PTN Recombinant Antibody (SAA1600).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human PTN Recombinant Antibody (SAA1600) (HB853013)

Overview

Images

References

Datasheet

SDS

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