Anti-PTN Polyclonal Antibody (HB853014)

Name: Anti-PTN Polyclonal Antibody
Brand: abinScience
SKU: HB853014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HB853014

Description

Anti-PTN Polyclonal Antibody (HB853014) is a rabbit polyclonal antibody detecting PTN in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Chicken.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human PTN (Gly33-Asp168).

Target

Heparin-binding neurite outgrowth-promoting factor 1, Heparin-binding brain mitogen, PTN, HBGF-8, Pleiotrophin, Heparin-binding growth factor 8, HBBM, HBNF-1, NEGF1, Osteoblast-specific factor 1, HBNF, HBNF1, Heparin-binding growth-associated molecule, HB-GAM, OSF-1, Heparin-binding neurite outgrowth-promoting factor

Purification

Purified by antigen affinity column.

Accession

P21246

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Pleiotrophin (PTN) is a ~18 kDa protein. Secreted growth factor that mediates its signal through cell-surface proteoglycan and non-proteoglycan receptors. Binds cell-surface proteoglycan receptor via their chondroitin sulfate (CS) groups. Thereby regulates many processes like cell proliferation, cell survival, cell growth, cell differentiation and cell migration in several tissues namely neuron and bone. Also plays a role in synaptic plasticity and learning-related behavior by inhibiting long-term synaptic potentiation. Binds PTPRZ1, leading to neutralization of the negative charges of the CS chains of PTPRZ1, inducing PTPRZ1 clustering, thereby causing the dimerization and inactivation of its phosphatase activity leading to increased tyrosine phosphorylation of each of the PTPRZ1 substrates like ALK, CTNNB1 or AFAP1L2 in order to activate the PI3K-AKT pathway.

1. Stoica, GE. et al. (2001) The Journal of biological chemistry 276, 16772-9. PMID: 11278720

2. Fukada, M. et al. (2006) FEBS letters 580, 4051-6. PMID: 16814777

3. Mikelis, C. et al. (2009) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 23, 1459-69. PMID: 19141530

4. Ryan, E. et al. (2016) The FEBS journal 283, 1488-503. PMID: 26896299

5. Kuboyama, K. et al. (2016) The Journal of biological chemistry 291, 18117-28. PMID: 27445335

6. Wellstein, A. et al. (1992) The Journal of biological chemistry 267, 2582-7. PMID: 1733956

7. Kretschmer, PJ. et al. (1991) Growth factors (Chur, Switzerland) 5, 99-114. PMID: 1768439

8. Imai, S. et al. (2009) Bone 44, 785-94. PMID: 19442624

Note

For research use only.

Images

References

Datasheet

Anti-PTN Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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Anti-PTN Polyclonal Antibody (HB853014)

Overview

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References

Datasheet

SDS

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