Anti-RAB5A Polyclonal Antibody (HB244014)

Name: Anti-RAB5A Polyclonal Antibody
Brand: abinScience
SKU: HB244014
Price: 168 USD
Availability: InStock

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50ug
100ug

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Referencias
Datasheet
SDS

Vista general

Número de catálogo

HB244014

Descripción

Anti-RAB5A Polyclonal Antibody (HB244014) is a rabbit polyclonal antibody detecting RAB5A in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Dog.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactividad de especies

Human, Mouse, Rat

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human RAB5A (Asp136-Asn215).

Objetivo

Ras-related protein Rab-5A, RAB5A, RAB5

Purificación

Purified by antigen affinity column.

Número de acceso

P20339

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Ras-related protein Rab-5A (RAB5A) is a ~23 kDa protein. The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes and involved in early endocytic trafficking. Required for EEA1 recruitment to early endosomes. Recruits FERRY complex to early endosomes, where FERRY links early endosomes with a subgroup of mRNAs to enable mRNA intracellular distribution.

1. Hoffenberg, S. et al. (2000) The Journal of biological chemistry 275, 24661-9. PMID: 10818110

2. Fouraux, MA. et al. (2004) Molecular biology of the cell 15, 611-24. PMID: 14617813

3. Zhu, G. et al. (2004) Nature structural & molecular biology 11, 975-83. PMID: 15378032

4. Haas, AK. et al. (2005) Nature cell biology 7, 887-93. PMID: 16086013

5. Hunker, CM. et al. (2006) Biochemical and biophysical research communications 340, 967-75. PMID: 16410077

6. Fuchs, E. et al. (2007) The Journal of cell biology 177, 1133-43. PMID: 17562788

7. Quentin, D. et al. (2023) Molecular cell 83, 1856-1871.e9. PMID: 37267906

8. Gauthier-Campbell, C. et al. (2004) Molecular biology of the cell 15, 2205-17. PMID: 14978216

9. Baietti, MF. et al. (2012) Nature cell biology 14, 677-85. PMID: 22660413

Nota

For research use only.

Imágenes

Referencias

Datasheet

Anti-RAB5A Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

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