Human RAB5A Recombinant Protein (N-His) (HB244012)

Name: Human RAB5A Recombinant Protein (N-His)
Brand: abinScience
SKU: HB244012
Price: 211 USD
Availability: InStock

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Catalog No.	HB244012
Description	Recombinant Human RAB5A Protein, N-His (HB244012) expressed in E. coli, spanning Asp136-Asn215. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P20339
Protein length	Asp136-Asn215
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	11.08 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Ras-related protein Rab-5A, RAB5A, RAB5
Background	Ras-related protein Rab-5A (RAB5A) is a ~23 kDa protein. The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes and involved in early endocytic trafficking. Required for EEA1 recruitment to early endosomes. Recruits FERRY complex to early endosomes, where FERRY links early endosomes with a subgroup of mRNAs to enable mRNA intracellular distribution. 1. Hoffenberg, S. et al. (2000) The Journal of biological chemistry 275, 24661-9. PMID: 10818110 2. Fouraux, MA. et al. (2004) Molecular biology of the cell 15, 611-24. PMID: 14617813 3. Zhu, G. et al. (2004) Nature structural & molecular biology 11, 975-83. PMID: 15378032 4. Haas, AK. et al. (2005) Nature cell biology 7, 887-93. PMID: 16086013 5. Hunker, CM. et al. (2006) Biochemical and biophysical research communications 340, 967-75. PMID: 16410077 6. Fuchs, E. et al. (2007) The Journal of cell biology 177, 1133-43. PMID: 17562788 7. Quentin, D. et al. (2023) Molecular cell 83, 1856-1871.e9. PMID: 37267906 8. Gauthier-Campbell, C. et al. (2004) Molecular biology of the cell 15, 2205-17. PMID: 14978216 9. Baietti, MF. et al. (2012) Nature cell biology 14, 677-85. PMID: 22660413
Note	For research use only.

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SDS-PAGE for Recombinant Human RAB5A Protein, N-His

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Datasheet

Human RAB5A Recombinant Protein (N-His).pdf

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Human RAB5A Recombinant Protein (N-His) (HB244012)

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