Anti-RAB5A Polyclonal Antibody (HB244014)

Name: Anti-RAB5A Polyclonal Antibody
Brand: abinScience
SKU: HB244014
Price: 168 USD
Availability: InStock

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Aperçu

Numéro de catalogue

HB244014

Description

Anti-RAB5A Polyclonal Antibody (HB244014) is a rabbit polyclonal antibody detecting RAB5A in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Dog.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human RAB5A (Asp136-Asn215).

Cible

Ras-related protein Rab-5A, RAB5A, RAB5

Purification

Purified by antigen affinity column.

Numéro d'accès

P20339

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Ras-related protein Rab-5A (RAB5A) is a ~23 kDa protein. The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes and involved in early endocytic trafficking. Required for EEA1 recruitment to early endosomes. Recruits FERRY complex to early endosomes, where FERRY links early endosomes with a subgroup of mRNAs to enable mRNA intracellular distribution.

1. Hoffenberg, S. et al. (2000) The Journal of biological chemistry 275, 24661-9. PMID: 10818110

2. Fouraux, MA. et al. (2004) Molecular biology of the cell 15, 611-24. PMID: 14617813

3. Zhu, G. et al. (2004) Nature structural & molecular biology 11, 975-83. PMID: 15378032

4. Haas, AK. et al. (2005) Nature cell biology 7, 887-93. PMID: 16086013

5. Hunker, CM. et al. (2006) Biochemical and biophysical research communications 340, 967-75. PMID: 16410077

6. Fuchs, E. et al. (2007) The Journal of cell biology 177, 1133-43. PMID: 17562788

7. Quentin, D. et al. (2023) Molecular cell 83, 1856-1871.e9. PMID: 37267906

8. Gauthier-Campbell, C. et al. (2004) Molecular biology of the cell 15, 2205-17. PMID: 14978216

9. Baietti, MF. et al. (2012) Nature cell biology 14, 677-85. PMID: 22660413

Note

For research use only.

Images

Reférences

Fiche technique

Anti-RAB5A Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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