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Anti-SARS-CoV-2 Nucleoprotein Recombinant Nanobody (SAA0961) (VK805043)

Anti-SARS-CoV-2 Nucleoprotein Recombinant Nanobody (SAA0961)
Anti-SARS-CoV-2 Nucleoprotein Recombinant Nanobody (SAA0961)
Anti-SARS-CoV-2 Nucleoprotein Recombinant Nanobody (SAA0961)
Anti-SARS-CoV-2 Nucleoprotein Recombinant Nanobody (SAA0961)
Precio(USD):
Especificación:
  • 50ug
  • 100ug
  • 1mg
Número:
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Vista general
Número de catálogoVK805043
Descripción
Anti-SARS-CoV-2 NP/Nucleoprotein Nanobody (SAA0961) [SAA0961] (VK805043) is a alpaca monoclonal antibody detecting Nucleoprotein in ELISA.
Reactividad de especiesSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
AplicacionesELISA
Especie huéspedAlpaca
ClonalidadMonoclonal
IsotipoVHH-Avi-8His-Cys-tag
ID de clonSAA0961
Objetivo Nucleoprotein, N, Nucleocapsid protein, NC, Protein N, N
Nivel de endotoxinas Please contact with the lab for this information.
Pureza >95% as determined by SDS-PAGE.
Purificación Purified by Nickel column
Número de acceso P0DTC9
Forma Liquid
Buffer de almacenamiento 0.01M PBS pH 7.4

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Reconstitución Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Información de uso del producto
Aplicación Dilución
ELISA 1:2000-1:20000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Nucleoprotein (NP) is a ~45 kDa protein. Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Attenuates the stress granules formation by reducing host G3BP1 access to host mRNAs under stress conditions.

1. Dinesh, DC. et al. (2020) PLoS pathogens 16, e1009100. PMID: 33264373
2. Nabeel-Shah, S. et al. (2022) iScience 25, 103562. PMID: 34901782
3. Dolliver, SM. et al. (2022) PLoS pathogens 18, e1011041. PMID: 36534661
Nota For research use only.
Imágenes
  • Anti-SARS-CoV-2 Nucleoprotein Recombinant Nanobody (SAA0961)

    SDS-PAGE

    SDS-PAGE for Anti-SARS-CoV-2 NP/Nucleoprotein Nanobody (SAA0961)

  • Anti-SARS-CoV-2 Nucleoprotein Recombinant Nanobody (SAA0961)

    Immunofluorescence

    Immunofluorescence staining of Hepg2 with Alpaca monoclonal antibody (20ug/ml, overnight at 4°C), followed by Alexa Fluor-488-conjugated secondary antibody (6.5ug/ml, 50minutes at room temperature). The target protein was detected in the Cell membrane.

Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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