Anti-SARS-CoV-2 Nucleoprotein Recombinant Nanobody (SAA0961) (VK805043)

Name: Anti-SARS-CoV-2 Nucleoprotein Recombinant Nanobody (SAA0961)
Brand: abinScience
SKU: VK805043
Price: 191 USD
Availability: InStock

データシート製品共有情報コピー

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50ug
100ug
1mg

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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号

VK805043

説明

Anti-SARS-CoV-2 NP/Nucleoprotein Nanobody (SAA0961) [SAA0961] (VK805043) is a alpaca monoclonal antibody detecting Nucleoprotein in ELISA.

種反応性

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)

アプリケーション

ELISA

宿主種

Alpaca

クローン性

Monoclonal

アイソタイプ

VHH-Avi-8His-Cys-tag

クローンID

SAA0961

ターゲット

Nucleoprotein, N, Nucleocapsid protein, NC, Protein N, N

エンドトキシンレベル

Please contact with the lab for this information.

純度

>95% as determined by SDS-PAGE.

精製

Purified by Nickel column

アクセッション番号

P0DTC9

形態

Liquid

保存バッファー

0.01M PBS pH 7.4

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

再構成

Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.

製品使用情報

アプリケーション	希釈
ELISA	1:2000-1:20000

安定性と保存

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Nucleoprotein (NP) is a ~45 kDa protein. Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Attenuates the stress granules formation by reducing host G3BP1 access to host mRNAs under stress conditions.

1. Dinesh, DC. et al. (2020) PLoS pathogens 16, e1009100. PMID: 33264373

2. Nabeel-Shah, S. et al. (2022) iScience 25, 103562. PMID: 34901782

3. Dolliver, SM. et al. (2022) PLoS pathogens 18, e1011041. PMID: 36534661

注意事項

For research use only.

イメージ

SDS-PAGE

SDS-PAGE for Anti-SARS-CoV-2 NP/Nucleoprotein Nanobody (SAA0961)
Immunofluorescence

Immunofluorescence staining of Hepg2 with Alpaca monoclonal antibody (20ug/ml, overnight at 4°C), followed by Alexa Fluor-488-conjugated secondary antibody (6.5ug/ml, 50minutes at room temperature). The target protein was detected in the Cell membrane.