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Каталожный номерHB182014
Описание
Anti-SFN Polyclonal Antibody (HB182014) is a rabbit polyclonal antibody detecting SFN in ELISA, IHC, WB. Suitable for Human, Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Реактивность видовHuman, Mouse
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant Human SFN (Met1-Ser248).
Цель Stratifin, Epithelial cell marker protein 1, HME1, 14-3-3 protein sigma, SFN
Очистка Purified by antigen affinity column.
Номер доступа P31947
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон

14-3-3 protein sigma (SFN) is a ~27 kDa protein. Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Promotes cytosolic retention of GBP1 GTPase by binding to phosphorylated GBP1, thereby inhibiting the innate immune response. Also acts as a TP53/p53-regulated inhibitor of G2/M progression.

1. Wilker, EW. et al. (2005) The Journal of biological chemistry 280, 18891-8. PMID: 15731107
2. Rose, R. et al. (2012) Journal of structural biology 180, 65-72. PMID: 22634725
3. Stevers, LM. et al. (2017) The Biochemical journal 474, 1273-1287. PMID: 28202711
4. Fisch, D. et al. (2023) Science (New York, N.Y.) 382, eadg2253. PMID: 37797010
7. Rietscher, K. et al. (2018) Journal of cell science 131. PMID: 29678907
Примечание For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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