Human SFN Recombinant Protein (N-His) (HB182012)

Name: Human SFN Recombinant Protein (N-His)
Brand: abinScience
SKU: HB182012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HB182012
Description	Recombinant Human SFN Protein, N-His (HB182012) expressed in E. coli, spanning Met1-Ser248. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P31947
Protein length	Met1-Ser248
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	29.94 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Stratifin, Epithelial cell marker protein 1, HME1, 14-3-3 protein sigma, SFN
Background	14-3-3 protein sigma (SFN) is a ~27 kDa protein. Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Promotes cytosolic retention of GBP1 GTPase by binding to phosphorylated GBP1, thereby inhibiting the innate immune response. Also acts as a TP53/p53-regulated inhibitor of G2/M progression. 1. Wilker, EW. et al. (2005) The Journal of biological chemistry 280, 18891-8. PMID: 15731107 2. Rose, R. et al. (2012) Journal of structural biology 180, 65-72. PMID: 22634725 3. Stevers, LM. et al. (2017) The Biochemical journal 474, 1273-1287. PMID: 28202711 4. Fisch, D. et al. (2023) Science (New York, N.Y.) 382, eadg2253. PMID: 37797010 7. Rietscher, K. et al. (2018) Journal of cell science 131. PMID: 29678907
Note	For research use only.

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SDS-PAGE for Recombinant Human SFN Protein, N-His

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Datasheet

Human SFN Recombinant Protein (N-His).pdf

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Human SFN Recombinant Protein (N-His) (HB182012)

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