Anti-Staphylococcus aureus Alpha-toxin/hly/AT Recombinant Antibody (LTM14) (JN998033)

Name: Anti-Staphylococcus aureus Alpha-toxin/hly/AT Recombinant Antibody (LTM14)
Brand: abinScience
SKU: JN998033
Price: 126 USD
Availability: InStock

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Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

JN998033

Reactivité des espèces

Staphylococcus aureus

Applications

ELISA, SPR

Espèce hôte

Mouse

Clonalité

Monoclonal

Isotype

IgG2a

ID de clone

LTM14

Cible

hly, hla, Alpha-hemolysin, Alpha-toxin, Alpha-HL, Staphylococcus aureus

Niveau d'endotoxines

Please contact with the lab for this information.

Pureté

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Numéro d'accès

P09616

RRID

Anti-Staphylococcus aureus Alpha-toxin/hly/AT Antibody (LTM14) (abinScience Cat# JN998033, RRID:AB_3729027)
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Forme

Liquid

Tampon de stockage

0.01M PBS pH 7.4

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:2000-1:20000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Contexte

Alpha-hemolysin (Alpha-toxin/hly) is a ~35 kDa protein. Alpha-toxin binds to the membrane of eukaryotic cells (particularly red blood cells, RBC) forming pores, resulting in hemolysis, with the release of low-molecular weight molecules leading to eventual osmotic RBC lysis. Human RBCs bind much less alpha-toxin than do rabbit RBCs. Heptamer oligomerization and pore formation is required for lytic activity.

1. Walker, B. et al. (1992) The Journal of biological chemistry 267, 10902-9. PMID: 1587866

2. Wilke, GA. et al. (2010) Proceedings of the National Academy of Sciences of the United States of America 107, 13473-8. PMID: 20624979

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Staphylococcus aureus Alpha-toxin/hly/AT Antibody (LTM14)
Western blot

Western blot analysis was performed using anti-/ monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant Staphylococcus aureus Alpha-toxin/hly(Catalog No: JN998012)
Lane 2: negative control