Anti-Streptococcus pyogenes Cas9 Polyclonal Antibody (JN045014)

Name: Anti-Streptococcus pyogenes Cas9 Polyclonal Antibody
Brand: abinScience
SKU: JN045014
Price: 168 USD
Availability: InStock

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Aperçu

Numéro de catalogue

JN045014

Description

Anti-Streptococcus pyogenes Cas9 Polyclonal Antibody (JN045014) is a rabbit polyclonal antibody detecting cas9 in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactivité des espèces

Streptococcus pyogenes

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Streptococcus pyogenes Cas9 (Met1-Asp1368).

Cible

CRISPR-associated endonuclease Cas9/Csn1, 3.1.-.-, SpCas9, SpyCas9, cas9, csn1, SPy_1046

Purification

Purified by antigen affinity column.

Numéro d'accès

Q99ZW2

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

CRISPR-associated endonuclease Cas9/Csn1 is a ~158 kDa protein. CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA; Cas9 only stabilizes the pre-crRNA:tracrRNA interaction and has no catalytic function in RNA processing.

1. Deltcheva, E. et al. (2011) Nature 471, 602-7. PMID: 21455174

2. Fonfara, I. et al. (2014) Nucleic acids research 42, 2577-90. PMID: 24270795

3. Sternberg, SH. et al. (2014) Nature 507, 62-7. PMID: 24476820

Note

For research use only.

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