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Recombinant Streptococcus pyogenes Cas9 Protein, N-His (JN045052)

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Recombinant Streptococcus pyogenes Cas9 Protein, N-His

Recombinant Streptococcus pyogenes Cas9 Protein, N-His

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Datasheet

Overview

Catalog No.	JN045052
Description	Recombinant Streptococcus pyogenes Cas9 Protein, N-His (JN045052) expressed in E. coli, spanning Met1-Asp1368. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q99ZW2
Protein length	Met1-Asp1368
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Streptococcus pyogenes
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	161.84 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	type II CRISPR RNA-guided endonuclease Cas9, cas9
Background	CRISPR-associated endonuclease Cas9/Csn1 is a ~158 kDa protein. CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA; Cas9 only stabilizes the pre-crRNA:tracrRNA interaction and has no catalytic function in RNA processing. 1. Deltcheva, E. et al. (2011) Nature 471, 602-7. PMID: 21455174 2. Fonfara, I. et al. (2014) Nucleic acids research 42, 2577-90. PMID: 24270795 3. Sternberg, SH. et al. (2014) Nature 507, 62-7. PMID: 24476820
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Streptococcus pyogenes Cas9 Protein, N-His

References

Datasheet

Recombinant Streptococcus pyogenes Cas9 Protein, N-His.pdf

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Recombinant Streptococcus pyogenes Cas9 Protein, N-His