Anti-Streptococcus pyogenes Cas9 Polyclonal Antibody (JN045014)

Name: Anti-Streptococcus pyogenes Cas9 Polyclonal Antibody
Brand: abinScience
SKU: JN045014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

JN045014

Description

Anti-Streptococcus pyogenes Cas9 Polyclonal Antibody (JN045014) is a rabbit polyclonal antibody detecting cas9 in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Streptococcus pyogenes

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Streptococcus pyogenes Cas9 (Met1-Asp1368).

Target

CRISPR-associated endonuclease Cas9/Csn1, 3.1.-.-, SpCas9, SpyCas9, cas9, csn1, SPy_1046

Purification

Purified by antigen affinity column.

Accession

Q99ZW2

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

CRISPR-associated endonuclease Cas9/Csn1 is a ~158 kDa protein. CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA; Cas9 only stabilizes the pre-crRNA:tracrRNA interaction and has no catalytic function in RNA processing.

1. Deltcheva, E. et al. (2011) Nature 471, 602-7. PMID: 21455174

2. Fonfara, I. et al. (2014) Nucleic acids research 42, 2577-90. PMID: 24270795

3. Sternberg, SH. et al. (2014) Nature 507, 62-7. PMID: 24476820

Note

For research use only.

Images

References

Datasheet