Anti-Streptococcus pyogenes speB/Streptopain Polyclonal Antibody (JN886014)

Name: Anti-Streptococcus pyogenes speB/Streptopain Polyclonal Antibody
Brand: abinScience
SKU: JN886014
Price: 168 USD
Availability: InStock

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Referencias
Datasheet
SDS

Vista general

Número de catálogo

JN886014

Marca

abinScience

Descripción

Anti-Streptococcus pyogenes speB/Streptopain Polyclonal Antibody (JN886014) is a rabbit polyclonal antibody detecting Streptopain in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactividad de especies

Streptococcus pyogenes

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Streptococcus pyogenes speB/Streptopain (Gln146-Pro398).

Objetivo

Streptopain, 3.4.22.10, Exotoxin type B, SPE B, Streptococcal cysteine proteinase, Streptococcus peptidase A, SPP, speB

Purificación

Purified by antigen affinity column.

Número de acceso

P0C0J0

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Streptopain (speB) is a ~43 kDa protein. Cysteine protease that acts as a key streptococcal virulence factor by cleaving host proteins involved in immune response. Triggers inflammation by mediating cleavage of host proteins, which can both promote host pathogenesis by triggering sterile inflammation and/or restrict streptococcal infection, depending on host immune statue and infection site. Cleaves host gasdermin-A (GSDMA) in epithelial cells, promoting GSDMA activation and formation of gasdermin pores, triggering pyroptosis. Pyroptosis triggers the elimination of the infected skin cell, depriving the pathogen of its protective niche, while inducing an inflammatory response. This ultimately prevents bacterial penetration of the epithelial barrier and a subsequent systemic dissemination of the pathogen.

1. Doran, JD. et al. (1999) European journal of biochemistry 263, 145-51. PMID: 10429198

2. Nomizu, M. et al. (2001) The Journal of biological chemistry 276, 44551-6. PMID: 11553627

3. Yu, CE. et al. (1991) Infection and immunity 59, 211-5. PMID: 1987034

4. González-Páez, GE. et al. (2012) The Journal of biological chemistry 287, 24412-26. PMID: 22645124

5. Musser, JM. et al. (1996) Infection and immunity 64, 1913-7. PMID: 8675287

6. Lukomski, S. et al. (1997) The Journal of clinical investigation 99, 2574-80. PMID: 9169486

7. Kuo, CF. et al. (1999) Infection and immunity 67, 126-30. PMID: 9864206

8. Kapur, V. et al. (1993) Proceedings of the National Academy of Sciences of the United States of America 90, 7676-80. PMID: 7689226

9. Kapur, V. et al. (1993) Microbial pathogenesis 15, 327-46. PMID: 7516997

Nota

For research use only.

Imágenes

Referencias

Datasheet

Anti-Streptococcus pyogenes speB/Streptopain Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo