ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Streptococcus pyogenes
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
41.21 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names
Streptopain, 3.4.22.10, Exotoxin type B, Group A streptococcal cysteine protease, Streptococcal cysteine proteinase, SPE B, Streptococcus peptidase A, SPP, speB
Background
Streptopain (speB) is a ~43 kDa protein. Cysteine protease that acts as a key streptococcal virulence factor by cleaving host proteins involved in immune response. Triggers inflammation by mediating cleavage of host proteins, which can both promote host pathogenesis by triggering sterile inflammation and/or restrict streptococcal infection, depending on host immune statue and infection site. Cleaves host gasdermin-A (GSDMA) in epithelial cells, promoting GSDMA activation and formation of gasdermin pores, triggering pyroptosis. Pyroptosis triggers the elimination of the infected skin cell, depriving the pathogen of its protective niche, while inducing an inflammatory response. This ultimately prevents bacterial penetration of the epithelial barrier and a subsequent systemic dissemination of the pathogen.
1. Doran, JD. et al. (1999) European journal of biochemistry 263, 145-51. PMID: 10429198
2. Nomizu, M. et al. (2001) The Journal of biological chemistry 276, 44551-6. PMID: 11553627
3. Yu, CE. et al. (1991) Infection and immunity 59, 211-5. PMID: 1987034
4. González-Páez, GE. et al. (2012) The Journal of biological chemistry 287, 24412-26. PMID: 22645124
5. Musser, JM. et al. (1996) Infection and immunity 64, 1913-7. PMID: 8675287
6. Lukomski, S. et al. (1997) The Journal of clinical investigation 99, 2574-80. PMID: 9169486
7. Kuo, CF. et al. (1999) Infection and immunity 67, 126-30. PMID: 9864206
8. Kapur, V. et al. (1993) Proceedings of the National Academy of Sciences of the United States of America 90, 7676-80. PMID: 7689226
9. Kapur, V. et al. (1993) Microbial pathogenesis 15, 327-46. PMID: 7516997
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Streptococcus pyogenes speB/Streptopain Protein, N-His-SUMO & C-Strep
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