Anti-Streptococcus pyogenes speB/Streptopain Polyclonal Antibody (JN886014)

Name: Anti-Streptococcus pyogenes speB/Streptopain Polyclonal Antibody
Brand: abinScience
SKU: JN886014
Price: 168 USD
Availability: InStock

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SDS

Aperçu

Numéro de catalogue

JN886014

Marque

abinScience

Description

Anti-Streptococcus pyogenes speB/Streptopain Polyclonal Antibody (JN886014) is a rabbit polyclonal antibody detecting Streptopain in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactivité des espèces

Streptococcus pyogenes

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Streptococcus pyogenes speB/Streptopain (Gln146-Pro398).

Cible

Streptopain, 3.4.22.10, Exotoxin type B, SPE B, Streptococcal cysteine proteinase, Streptococcus peptidase A, SPP, speB

Purification

Purified by antigen affinity column.

Numéro d'accès

P0C0J0

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Streptopain (speB) is a ~43 kDa protein. Cysteine protease that acts as a key streptococcal virulence factor by cleaving host proteins involved in immune response. Triggers inflammation by mediating cleavage of host proteins, which can both promote host pathogenesis by triggering sterile inflammation and/or restrict streptococcal infection, depending on host immune statue and infection site. Cleaves host gasdermin-A (GSDMA) in epithelial cells, promoting GSDMA activation and formation of gasdermin pores, triggering pyroptosis. Pyroptosis triggers the elimination of the infected skin cell, depriving the pathogen of its protective niche, while inducing an inflammatory response. This ultimately prevents bacterial penetration of the epithelial barrier and a subsequent systemic dissemination of the pathogen.

1. Doran, JD. et al. (1999) European journal of biochemistry 263, 145-51. PMID: 10429198

2. Nomizu, M. et al. (2001) The Journal of biological chemistry 276, 44551-6. PMID: 11553627

3. Yu, CE. et al. (1991) Infection and immunity 59, 211-5. PMID: 1987034

4. González-Páez, GE. et al. (2012) The Journal of biological chemistry 287, 24412-26. PMID: 22645124

5. Musser, JM. et al. (1996) Infection and immunity 64, 1913-7. PMID: 8675287

6. Lukomski, S. et al. (1997) The Journal of clinical investigation 99, 2574-80. PMID: 9169486

7. Kuo, CF. et al. (1999) Infection and immunity 67, 126-30. PMID: 9864206

8. Kapur, V. et al. (1993) Proceedings of the National Academy of Sciences of the United States of America 90, 7676-80. PMID: 7689226

9. Kapur, V. et al. (1993) Microbial pathogenesis 15, 327-46. PMID: 7516997

Note

For research use only.

Images

Reférences

Fiche technique

Anti-Streptococcus pyogenes speB/Streptopain Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

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