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Anti-SV40 Large T Antigen Polyclonal Antibody (VK099024)

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Anti-SV40 Large T Antigen Polyclonal Antibody
Anti-SV40 Large T Antigen Polyclonal Antibody
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Especificación:
  • 50ug
  • 100ug
Número:
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Vista general
Número de catálogoVK099024
Descripción
Anti-SV40 Large T Antigen Polyclonal Antibody (VK099024) is a rabbit polyclonal antibody detecting Large T antigen in ELISA, IHC, WB. Suitable for Simian virus 40.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesSimian virus 40 (SV40)
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant SV40 Large T Antigen (Gly251-Asp627).
Objetivo Large T antigen, LT, LT-AG, 5.6.2.4, DNA 3'-5' helicase large T antigen
Nivel de endotoxinas Please contact with the lab for this information.
Pureza >95% as determined by SDS-PAGE.
Purificación Purified by antigen affinity column.
Número de acceso P03070
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000,IHC:1:50-1:500,WB:1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Large T antigen is a ~81 kDa protein. Isoform large T antigen is a key early protein essential for both driving viral replication and inducing cellular transformation. Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle and by autoregulating the synthesis of viral early mRNA. Displays highly oncogenic activities by corrupting the host cellular checkpoint mechanisms that guard cell division and the transcription, replication, and repair of DNA. Participates in the modulation of cellular gene expression preceeding viral DNA replication. This step involves binding to host key cell cycle regulators retinoblastoma protein RB1/pRb and TP53.

1. Goetz, GS. et al. (1988) The Journal of biological chemistry 263, 383-92. PMID: 2826443
2. Wiekowski, M. et al. (1988) The Journal of biological chemistry 263, 436-42. PMID: 2826446
Nota For research use only
Imágenes
  • Anti-SV40 Large T Antigen Polyclonal Antibody

    Western blot

    Lane 1: recombinant SV40 Large T Antigen (Catalog No: VK099022)

Referencias
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-SV40 Large T Antigen Polyclonal Antibody

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