SV40 Large T Antigen Recombinant Protein (N-His) (VK099022)

Name: SV40 Large T Antigen Recombinant Protein (N-His)
Brand: abinScience
SKU: VK099022
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	VK099022
Description	Recombinant SV40 Large T Antigen Protein, N-His (VK099022) expressed in E. coli, spanning Gly251-Asp627. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P03070
Protein length	Gly251-Asp627
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Simian virus 40 (SV40)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	45.78 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Large T antigen, LT, LT-AG, 5.6.2.4, DNA 3'-5' helicase large T antigen
Background	Large T antigen is a ~81 kDa protein. Isoform large T antigen is a key early protein essential for both driving viral replication and inducing cellular transformation. Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle and by autoregulating the synthesis of viral early mRNA. Displays highly oncogenic activities by corrupting the host cellular checkpoint mechanisms that guard cell division and the transcription, replication, and repair of DNA. Participates in the modulation of cellular gene expression preceeding viral DNA replication. This step involves binding to host key cell cycle regulators retinoblastoma protein RB1/pRb and TP53. 1. Goetz, GS. et al. (1988) The Journal of biological chemistry 263, 383-92. PMID: 2826443 2. Wiekowski, M. et al. (1988) The Journal of biological chemistry 263, 436-42. PMID: 2826446
Note	For research use only

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SDS-PAGE

SDS-PAGE for Recombinant SV40 Large T Antigen Protein, N-His

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Datasheet

SV40 Large T Antigen Recombinant Protein (N-His).pdf

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SV40 Large T Antigen Recombinant Protein (N-His) (VK099022)

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