Anti-SV40 Large T Antigen Polyclonal Antibody (VK099024)

Name: Anti-SV40 Large T Antigen Polyclonal Antibody
Brand: abinScience
SKU: VK099024
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

VK099024

Description

Anti-SV40 Large T Antigen Polyclonal Antibody (VK099024) is a rabbit polyclonal antibody detecting Large T antigen in ELISA, IHC, WB. Suitable for Simian virus 40.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Simian virus 40 (SV40)

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant SV40 Large T Antigen (Gly251-Asp627).

Target

Large T antigen, LT, LT-AG, 5.6.2.4, DNA 3'-5' helicase large T antigen

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P03070

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000,IHC:1:50-1:500,WB:1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Large T antigen is a ~81 kDa protein. Isoform large T antigen is a key early protein essential for both driving viral replication and inducing cellular transformation. Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle and by autoregulating the synthesis of viral early mRNA. Displays highly oncogenic activities by corrupting the host cellular checkpoint mechanisms that guard cell division and the transcription, replication, and repair of DNA. Participates in the modulation of cellular gene expression preceeding viral DNA replication. This step involves binding to host key cell cycle regulators retinoblastoma protein RB1/pRb and TP53.

1. Goetz, GS. et al. (1988) The Journal of biological chemistry 263, 383-92. PMID: 2826443

2. Wiekowski, M. et al. (1988) The Journal of biological chemistry 263, 436-42. PMID: 2826446

Note

For research use only

Images

Western blot

Lane 1: recombinant SV40 Large T Antigen (Catalog No: VK099022)

References

Datasheet

Anti-SV40 Large T Antigen Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-SV40 Large T Antigen Polyclonal Antibody (VK099024)

Overview

Images

References

Datasheet

SDS

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