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Anti-SV40 Large T Antigen Polyclonal Antibody (VK099024)

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Anti-SV40 Large T Antigen Polyclonal Antibody
Anti-SV40 Large T Antigen Polyclonal Antibody
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  • 100ug
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Aperçu
Numéro de catalogueVK099024
Description
Anti-SV40 Large T Antigen Polyclonal Antibody (VK099024) is a rabbit polyclonal antibody detecting Large T antigen in ELISA, IHC, WB. Suitable for Simian virus 40.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesSimian virus 40 (SV40)
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant SV40 Large T Antigen (Gly251-Asp627).
Cible Large T antigen, LT, LT-AG, 5.6.2.4, DNA 3'-5' helicase large T antigen
Niveau d'endotoxines Please contact with the lab for this information.
Pureté >95% as determined by SDS-PAGE.
Purification Purified by antigen affinity column.
Numéro d'accès P03070
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000,IHC:1:50-1:500,WB:1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Large T antigen is a ~81 kDa protein. Isoform large T antigen is a key early protein essential for both driving viral replication and inducing cellular transformation. Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle and by autoregulating the synthesis of viral early mRNA. Displays highly oncogenic activities by corrupting the host cellular checkpoint mechanisms that guard cell division and the transcription, replication, and repair of DNA. Participates in the modulation of cellular gene expression preceeding viral DNA replication. This step involves binding to host key cell cycle regulators retinoblastoma protein RB1/pRb and TP53.

1. Goetz, GS. et al. (1988) The Journal of biological chemistry 263, 383-92. PMID: 2826443
2. Wiekowski, M. et al. (1988) The Journal of biological chemistry 263, 436-42. PMID: 2826446
Note For research use only
Images
  • Anti-SV40 Large T Antigen Polyclonal Antibody

    Western blot

    Lane 1: recombinant SV40 Large T Antigen (Catalog No: VK099022)

Reférences
Fiche technique
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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