Anti-Human TNF-alpha Polyclonal Antibody (HF879014)

Name: Anti-Human TNF-alpha Polyclonal Antibody
Brand: abinScience
SKU: HF879014
Price: 168 USD
Availability: InStock

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SDS

Vista general

Número de catálogo

HF879014

Descripción

Anti-TNFa/TNF-alpha Polyclonal Antibody (HF879014) is a rabbit polyclonal antibody detecting TNF-alpha in ELISA, IHC, WB. Suitable for Human, Dog, Cat, Callithrix jacchus, and Equus caballus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactividad de especies

Human

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human TNFa/TNF-alpha (Val77-Leu233).

Objetivo

C-domain 1, C-domain 2, Cachectin, ICD1, ICD2, Intracellular domain 1, Intracellular domain 2, N-terminal fragment, NTF, TNF, TNF-a, TNF-alpha, TNFA, TNFSF2, Tumor necrosis factor, Tumor necrosis factor ligand superfamily member 2, Tumor necrosis factor, membrane form, Tumor necrosis factor, soluble form

Nivel de endotoxinas

Please contact with the lab for this information.

Pureza

>90% as determined by SDS-PAGE.

Purificación

Purified by antigen affinity column.

Número de acceso

P01375

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Tumor necrosis factor (TNFa/TNF-alpha) is a ~25 kDa protein. Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Up-regulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective. TNFa is the therapeutic target of adalimumab (Humira).

1. Nie, H. et al. (2013) Nature medicine 19, 322-8. PMID: 23396208

2. Friedmann, E. et al. (2006) Nature cell biology 8, 843-8. PMID: 16829952

3. Jinesh G, G. et al. (2012) Journal of leukocyte biology 92, 233-44. PMID: 22517918

4. Nakahara, H. et al. (2003) Arthritis and rheumatism 48, 1521-9. PMID: 12794819

Nota

For research use only.

Imágenes

Referencias

Datasheet