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Anti-USP14 Polyclonal Antibody (HX186014)

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Aperçu
Numéro de catalogueHX186014
Description
Anti-USP14 Polyclonal Antibody (HX186014) is a rabbit polyclonal antibody detecting USP14 in ELISA, IHC, WB. Suitable for Human, Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesHuman, Mouse
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human USP14 (Met1-Gln494).
Cible Deubiquitinating enzyme 14, TGT, Ubiquitin thioesterase 14, Ubiquitin carboxyl-terminal hydrolase 14, Ubiquitin-specific-processing protease 14, USP14
Purification Purified by antigen affinity column.
Numéro d'accès P54578
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Ubiquitin carboxyl-terminal hydrolase 14 (USP14) is a ~56 kDa protein. Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome. Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell. Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Also serves as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1.

1. Liu, D. et al. (2022) Proceedings of the National Academy of Sciences of the United States of America 119. PMID: 35145029
2. Koulich, E. et al. (2008) Molecular biology of the cell 19, 1072-82. PMID: 18162577
3. Kuo, CL. et al. (2017) Proceedings of the National Academy of Sciences of the United States of America 114, E3404-E3413. PMID: 28396413
4. Mines, MA. et al. (2009) The Journal of biological chemistry 284, 5742-52. PMID: 19106094
5. Nagai, A. et al. (2009) Biochemical and biophysical research communications 379, 995-1000. PMID: 19135427
6. Chen, M. et al. (2016) Molecular cell 64, 105-119. PMID: 27666593
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-USP14 Polyclonal Antibody

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