Human USP14 Recombinant Protein (N-His) (HX186012)

Name: Human USP14 Recombinant Protein (N-His)
Brand: abinScience
SKU: HX186012
Price: 211 USD
Availability: InStock

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Catalog No.	HX186012
Description	Recombinant Human USP14 Protein, N-His (HX186012) expressed in E. coli, spanning Met1-Gln494. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P54578
Protein length	Met1-Gln494
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	58.23 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Deubiquitinating enzyme 14, TGT, Ubiquitin thioesterase 14, Ubiquitin carboxyl-terminal hydrolase 14, Ubiquitin-specific-processing protease 14, USP14
Background	Ubiquitin carboxyl-terminal hydrolase 14 (USP14) is a ~56 kDa protein. Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome. Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell. Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Also serves as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. 1. Liu, D. et al. (2022) Proceedings of the National Academy of Sciences of the United States of America 119. PMID: 35145029 2. Koulich, E. et al. (2008) Molecular biology of the cell 19, 1072-82. PMID: 18162577 3. Kuo, CL. et al. (2017) Proceedings of the National Academy of Sciences of the United States of America 114, E3404-E3413. PMID: 28396413 4. Mines, MA. et al. (2009) The Journal of biological chemistry 284, 5742-52. PMID: 19106094 5. Nagai, A. et al. (2009) Biochemical and biophysical research communications 379, 995-1000. PMID: 19135427 6. Chen, M. et al. (2016) Molecular cell 64, 105-119. PMID: 27666593
Note	For research use only.

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SDS-PAGE for Recombinant Human USP14 Protein, N-His

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Datasheet

Human USP14 Recombinant Protein (N-His).pdf

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Human USP14 Recombinant Protein (N-His) (HX186012)

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