Anti-USP14 Polyclonal Antibody (HX186014)

Name: Anti-USP14 Polyclonal Antibody
Brand: abinScience
SKU: HX186014
Price: 168 USD
Availability: InStock

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Datasheet
SDS

Overview

Catalog No.

HX186014

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human USP14 (Met1-Gln494).

Target

Deubiquitinating enzyme 14, TGT, Ubiquitin thioesterase 14, Ubiquitin carboxyl-terminal hydrolase 14, Ubiquitin-specific-processing protease 14, USP14

Purification

Purified by antigen affinity column.

Accession

P54578

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Ubiquitin carboxyl-terminal hydrolase 14 (USP14) is a ~56 kDa protein. Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome. Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell. Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Also serves as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1.

1. Liu, D. et al. (2022) Proceedings of the National Academy of Sciences of the United States of America 119. PMID: 35145029

2. Koulich, E. et al. (2008) Molecular biology of the cell 19, 1072-82. PMID: 18162577

3. Kuo, CL. et al. (2017) Proceedings of the National Academy of Sciences of the United States of America 114, E3404-E3413. PMID: 28396413

4. Mines, MA. et al. (2009) The Journal of biological chemistry 284, 5742-52. PMID: 19106094

5. Nagai, A. et al. (2009) Biochemical and biophysical research communications 379, 995-1000. PMID: 19135427

6. Chen, M. et al. (2016) Molecular cell 64, 105-119. PMID: 27666593

Note

For research use only.

Images

References

Datasheet

Anti-USP14 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-USP14 Polyclonal Antibody (HX186014)

Overview

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References

Datasheet

SDS

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