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Anti-VAPA Polyclonal Antibody (HV508014)

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Aperçu
Numéro de catalogueHV508014
Description
Anti-VAPA Polyclonal Antibody (HV508014) is a rabbit polyclonal antibody detecting VAPA in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human VAPA (Met8-Met132).
Cible 33 kDa VAMP-associated protein, VAP-33, VAP33, VAPA, VAMP-associated protein A, VAMP-A, VAP-A, Vesicle-associated membrane protein-associated protein A
Purification Purified by antigen affinity column.
Numéro d'accès Q9P0L0
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Vesicle-associated membrane protein-associated protein A (VAPA) is a ~27 kDa protein. Endoplasmic reticulum (ER)-anchored protein that mediates the formation of contact sites between the ER and endosomes via interaction with FFAT motif-containing proteins such as STARD3 or WDR44. STARD3-VAPA interaction enables cholesterol transfer from the ER to endosomes. Via interaction with WDR44 participates in neosynthesized protein export. In addition, recruited to the plasma membrane through OSBPL3 binding. The OSBPL3-VAPA complex stimulates RRAS signaling which in turn attenuates integrin beta-1 (ITGB1) activation at the cell surface.

1. Lucken-Ardjomande Häsler, S. et al. (2020) The Journal of cell biology 219. PMID: 32344433
2. Di Mattia, T. et al. (2020) The EMBO journal 39, e104369. PMID: 33124732
3. Weber-Boyvat, M. et al. (2015) Experimental cell research 331, 278-91. PMID: 25447204
4. Lehto, M. et al. (2005) Experimental cell research 310, 445-62. PMID: 16143324
5. Weir, ML. et al. (2001) Biochemical and biophysical research communications 286, 616-21. PMID: 11511104
6. Saita, S. et al. (2009) The Journal of biological chemistry 284, 13766-13777. PMID: 19289470
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-VAPA Polyclonal Antibody

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