Human VAPA Recombinant Protein (N-His) (HV508012)

Name: Human VAPA Recombinant Protein (N-His)
Brand: abinScience
SKU: HV508012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HV508012
Description	Recombinant Human VAPA Protein, N-His (HV508012) expressed in E. coli, spanning Met8-Met132. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q9P0L0
Protein length	Met8-Met132
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	16.44 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	33 kDa VAMP-associated protein, VAP-33, VAP33, VAPA, VAMP-associated protein A, VAMP-A, VAP-A, Vesicle-associated membrane protein-associated protein A
Background	Vesicle-associated membrane protein-associated protein A (VAPA) is a ~27 kDa protein. Endoplasmic reticulum (ER)-anchored protein that mediates the formation of contact sites between the ER and endosomes via interaction with FFAT motif-containing proteins such as STARD3 or WDR44. STARD3-VAPA interaction enables cholesterol transfer from the ER to endosomes. Via interaction with WDR44 participates in neosynthesized protein export. In addition, recruited to the plasma membrane through OSBPL3 binding. The OSBPL3-VAPA complex stimulates RRAS signaling which in turn attenuates integrin beta-1 (ITGB1) activation at the cell surface. 1. Lucken-Ardjomande Häsler, S. et al. (2020) The Journal of cell biology 219. PMID: 32344433 2. Di Mattia, T. et al. (2020) The EMBO journal 39, e104369. PMID: 33124732 3. Weber-Boyvat, M. et al. (2015) Experimental cell research 331, 278-91. PMID: 25447204 4. Lehto, M. et al. (2005) Experimental cell research 310, 445-62. PMID: 16143324 5. Weir, ML. et al. (2001) Biochemical and biophysical research communications 286, 616-21. PMID: 11511104 6. Saita, S. et al. (2009) The Journal of biological chemistry 284, 13766-13777. PMID: 19289470
Note	For research use only.

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SDS-PAGE for Recombinant Human VAPA Protein, N-His

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Datasheet

Human VAPA Recombinant Protein (N-His).pdf

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Human VAPA Recombinant Protein (N-His) (HV508012)

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