Anti-VAPA Polyclonal Antibody (HV508014)

Name: Anti-VAPA Polyclonal Antibody
Brand: abinScience
SKU: HV508014
Price: 168 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD):

Spec:

50ug
100ug

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HV508014

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human VAPA (Met8-Met132).

Target

33 kDa VAMP-associated protein, VAP-33, VAP33, VAPA, VAMP-associated protein A, VAMP-A, VAP-A, Vesicle-associated membrane protein-associated protein A

Purification

Purified by antigen affinity column.

Accession

Q9P0L0

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Vesicle-associated membrane protein-associated protein A (VAPA) is a ~27 kDa protein. Endoplasmic reticulum (ER)-anchored protein that mediates the formation of contact sites between the ER and endosomes via interaction with FFAT motif-containing proteins such as STARD3 or WDR44. STARD3-VAPA interaction enables cholesterol transfer from the ER to endosomes. Via interaction with WDR44 participates in neosynthesized protein export. In addition, recruited to the plasma membrane through OSBPL3 binding. The OSBPL3-VAPA complex stimulates RRAS signaling which in turn attenuates integrin beta-1 (ITGB1) activation at the cell surface.

1. Lucken-Ardjomande Häsler, S. et al. (2020) The Journal of cell biology 219. PMID: 32344433

2. Di Mattia, T. et al. (2020) The EMBO journal 39, e104369. PMID: 33124732

3. Weber-Boyvat, M. et al. (2015) Experimental cell research 331, 278-91. PMID: 25447204

4. Lehto, M. et al. (2005) Experimental cell research 310, 445-62. PMID: 16143324

5. Weir, ML. et al. (2001) Biochemical and biophysical research communications 286, 616-21. PMID: 11511104

6. Saita, S. et al. (2009) The Journal of biological chemistry 284, 13766-13777. PMID: 19289470

Note

For research use only.

Images

References

Datasheet

Anti-VAPA Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-VAPA Polyclonal Antibody (HV508014)

Overview

Images

References

Datasheet

SDS

Quick access

Contact Us