Anti-WTAP Polyclonal Antibody (HC538014)

Name: Anti-WTAP Polyclonal Antibody
Brand: abinScience
SKU: HC538014
Price: 96 USD
Availability: InStock

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Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

HC538014

Reactividad de especies

Human, Mouse

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human WTAP (Met1-Asp150).

Objetivo

Wilms tumor 1-associating protein, Pre-mRNA-splicing regulator WTAP, hFL(2)D, WT1-associated protein, Female-lethal(2)D homolog, KIAA0105, WTAP

Purificación

Purified by antigen affinity column.

Número de acceso

Q15007

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Pre-mRNA-splicing regulator WTAP is a ~44 kDa protein. Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing. Required for accumulation of METTL3 and METTL14 to nuclear speckle. Acts as a mRNA splicing regulator. Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability. Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes.

1. Yue, Y. et al. (2018) Cell discovery 4, 10. PMID: 29507755

2. Liu, J. et al. (2014) Nature chemical biology 10, 93-5. PMID: 24316715

3. Ping, XL. et al. (2014) Cell research 24, 177-89. PMID: 24407421

4. Schwartz, S. et al. (2014) Cell reports 8, 284-96. PMID: 24981863

5. Ortega, A. et al. (2003) The Journal of biological chemistry 278, 3040-7. PMID: 12444081

6. Horiuchi, K. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 17278-83. PMID: 17088532

7. Small, TW. et al. (2006) Circulation research 99, 1338-46. PMID: 17095724

Nota

For research use only.

Imágenes

Western blot

Western blot analysis was performed using anti-WTAP polyclonal antibody at 1μg/ml on various samples.
Lane 1: Raw264.7 cell lysate
Lane 2: Jurkat cell lysate

Referencias

Datasheet

Anti-WTAP Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo