Human WTAP Recombinant Protein (N-His) (HC538012)

Name: Human WTAP Recombinant Protein (N-His)
Brand: abinScience
SKU: HC538012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HC538012
Description	Recombinant Human WTAP Protein, N-His (HC538012) expressed in E. coli, spanning Met1-Asp150. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q15007
Protein length	Met1-Asp150
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	19.81 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Wilms tumor 1-associating protein, Pre-mRNA-splicing regulator WTAP, hFL(2)D, WT1-associated protein, Female-lethal(2)D homolog, KIAA0105, WTAP
Background	Pre-mRNA-splicing regulator WTAP is a ~44 kDa protein. Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing. Required for accumulation of METTL3 and METTL14 to nuclear speckle. Acts as a mRNA splicing regulator. Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability. Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes. 1. Yue, Y. et al. (2018) Cell discovery 4, 10. PMID: 29507755 2. Liu, J. et al. (2014) Nature chemical biology 10, 93-5. PMID: 24316715 3. Ping, XL. et al. (2014) Cell research 24, 177-89. PMID: 24407421 4. Schwartz, S. et al. (2014) Cell reports 8, 284-96. PMID: 24981863 5. Ortega, A. et al. (2003) The Journal of biological chemistry 278, 3040-7. PMID: 12444081 6. Horiuchi, K. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 17278-83. PMID: 17088532 7. Small, TW. et al. (2006) Circulation research 99, 1338-46. PMID: 17095724
Note	For research use only.

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SDS-PAGE for Recombinant Human WTAP Protein, N-His

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Datasheet

Human WTAP Recombinant Protein (N-His).pdf

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Human WTAP Recombinant Protein (N-His) (HC538012)

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