Anti-WTAP Polyclonal Antibody (HC538014)

Name: Anti-WTAP Polyclonal Antibody
Brand: abinScience
SKU: HC538014
Price: 96 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD):

Spec:

20ug
50ug
100ug

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HC538014

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human WTAP (Met1-Asp150).

Target

Wilms tumor 1-associating protein, Pre-mRNA-splicing regulator WTAP, hFL(2)D, WT1-associated protein, Female-lethal(2)D homolog, KIAA0105, WTAP

Purification

Purified by antigen affinity column.

Accession

Q15007

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Pre-mRNA-splicing regulator WTAP is a ~44 kDa protein. Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing. Required for accumulation of METTL3 and METTL14 to nuclear speckle. Acts as a mRNA splicing regulator. Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability. Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes.

1. Yue, Y. et al. (2018) Cell discovery 4, 10. PMID: 29507755

2. Liu, J. et al. (2014) Nature chemical biology 10, 93-5. PMID: 24316715

3. Ping, XL. et al. (2014) Cell research 24, 177-89. PMID: 24407421

4. Schwartz, S. et al. (2014) Cell reports 8, 284-96. PMID: 24981863

5. Ortega, A. et al. (2003) The Journal of biological chemistry 278, 3040-7. PMID: 12444081

6. Horiuchi, K. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 17278-83. PMID: 17088532

7. Small, TW. et al. (2006) Circulation research 99, 1338-46. PMID: 17095724

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-WTAP polyclonal antibody at 1μg/ml on various samples.
Lane 1: Raw264.7 cell lysate
Lane 2: Jurkat cell lysate

References

Datasheet

Anti-WTAP Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-WTAP Polyclonal Antibody (HC538014)

Overview

Images

References

Datasheet

SDS

Quick access

Contact Us