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| カタログ番号 | HC538014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 種反応性 | Human, Mouse | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human WTAP (Met1-Asp150). | ||||||||
| ターゲット | Wilms tumor 1-associating protein, Pre-mRNA-splicing regulator WTAP, hFL(2)D, WT1-associated protein, Female-lethal(2)D homolog, KIAA0105, WTAP | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | Q15007 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Pre-mRNA-splicing regulator WTAP is a ~44 kDa protein. Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing. Required for accumulation of METTL3 and METTL14 to nuclear speckle. Acts as a mRNA splicing regulator. Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability. Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes. 1. Yue, Y. et al. (2018) Cell discovery 4, 10. PMID: 29507755 2. Liu, J. et al. (2014) Nature chemical biology 10, 93-5. PMID: 24316715 3. Ping, XL. et al. (2014) Cell research 24, 177-89. PMID: 24407421 4. Schwartz, S. et al. (2014) Cell reports 8, 284-96. PMID: 24981863 5. Ortega, A. et al. (2003) The Journal of biological chemistry 278, 3040-7. PMID: 12444081 6. Horiuchi, K. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 17278-83. PMID: 17088532 7. Small, TW. et al. (2006) Circulation research 99, 1338-46. PMID: 17095724 | ||||||||
| 注意事項 | For research use only. |
Western blot analysis was performed using anti-WTAP polyclonal antibody at 1μg/ml on various samples.
Lane 1: Raw264.7 cell lysate
Lane 2: Jurkat cell lysate
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