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Anti-Yersinia enterocolitica sctE/YopB Polyclonal Antibody (JN174014)

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Aperçu
Numéro de catalogueJN174014
Description
Anti-Yersinia enterocolitica sctE/YopB Polyclonal Antibody (JN174014) is a rabbit polyclonal antibody detecting sctE. Suitable for Yersinia pseudotuberculosis serotype I and Yersinia enterocolitica.
Highlights
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesYersinia pseudotuberculosis, Yersinia enterocolitica
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Yersinia enterocolitica sctE/YopB (Gly246-Val401).
Cible T3SS translocon protein SctE, Translocator YopB, Type 3 secretion system translocon protein SctE, Yersinia outer protein B, sctE, yopB
Niveau d'endotoxines Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Numéro d'accès P37131
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Contexte

Type 3 secretion system translocon protein SctE (YopB) is a ~41 kDa protein. Component of the type III secretion system (T3SS), also called injectisome, which is used to inject bacterial effector proteins into eukaryotic host cells. YopB/SctE and YopD/SctB are inserted into the host membrane where they form a pore and allow the translocation of effector proteins into the cytosol of target cells. Is an essential virulence determinant. Required for YopE translocation.

1. Neyt, C. et al. (1999) Molecular microbiology 33, 971-81. PMID: 10476031
2. Tardy, F. et al. (1999) The EMBO journal 18, 6793-9. PMID: 10581252
3. Lee, VT. et al. (1999) Molecular microbiology 31, 1619-29. PMID: 10209737
4. Nordfelth, R. et al. (2001) Infection and immunity 69, 3516-8. PMID: 11292787
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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