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Yersinia enterocolitica sctE/YopB Recombinant Protein (N-His) (JN174012)

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Overview
Catalog No.JN174012
Description
Recombinant Yersinia enterocolitica sctE/YopB Protein, N-His (JN174012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
  • E. coli Expression — High-yield, cost-effective production.
  • High Purity — >90% as determined by SDS-PAGE.
Expression systemE. coli
AccessionP37131
Protein lengthGly246-Val401
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
SpeciesYersinia enterocolitica
Nature Recombinant
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Form Lyophilized
Storage buffer Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesT3SS translocon protein SctE, Translocator YopB, Type 3 secretion system translocon protein SctE, Yersinia outer protein B, sctE, yopB
Background

Type 3 secretion system translocon protein SctE is a ~41 kDa protein. Component of the type III secretion system (T3SS), also called injectisome, which is used to inject bacterial effector proteins into eukaryotic host cells. YopB/SctE and YopD/SctB are inserted into the host membrane where they form a pore and allow the translocation of effector proteins into the cytosol of target cells. Is an essential virulence determinant. Required for YopE translocation.

1. Neyt, C. et al. (1999) Molecular microbiology 33, 971-81. PMID: 10476031
2. Tardy, F. et al. (1999) The EMBO journal 18, 6793-9. PMID: 10581252
3. Lee, VT. et al. (1999) Molecular microbiology 31, 1619-29. PMID: 10209737
4. Nordfelth, R. et al. (2001) Infection and immunity 69, 3516-8. PMID: 11292787
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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