

| Catalog No. | JN174012 |
|---|---|
| Description |
Recombinant Yersinia enterocolitica sctE/YopB Protein, N-His (JN174012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
|
| Expression system | E. coli |
| Accession | P37131 |
| Protein length | Gly246-Val401 |
| Applications | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Species | Yersinia enterocolitica |
| Nature | Recombinant |
| Endotoxin level | Please contact with the lab for this information. |
| Purity | >90% as determined by SDS-PAGE. |
| Form | Lyophilized |
| Storage buffer | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Reconstitution | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Shipping | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Alternate Names | T3SS translocon protein SctE, Translocator YopB, Type 3 secretion system translocon protein SctE, Yersinia outer protein B, sctE, yopB |
| Background | Type 3 secretion system translocon protein SctE is a ~41 kDa protein. Component of the type III secretion system (T3SS), also called injectisome, which is used to inject bacterial effector proteins into eukaryotic host cells. YopB/SctE and YopD/SctB are inserted into the host membrane where they form a pore and allow the translocation of effector proteins into the cytosol of target cells. Is an essential virulence determinant. Required for YopE translocation. 1. Neyt, C. et al. (1999) Molecular microbiology 33, 971-81. PMID: 10476031 2. Tardy, F. et al. (1999) The EMBO journal 18, 6793-9. PMID: 10581252 3. Lee, VT. et al. (1999) Molecular microbiology 31, 1619-29. PMID: 10209737 4. Nordfelth, R. et al. (2001) Infection and immunity 69, 3516-8. PMID: 11292787 |
| Note | For research use only |
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