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Anti-Yersinia enterocolitica sctE/YopB Polyclonal Antibody (JN174014)

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Overview
Catalog No.JN174014
Description
Anti-Yersinia enterocolitica sctE/YopB Polyclonal Antibody (JN174014) is a rabbit polyclonal antibody detecting sctE. Suitable for Yersinia pseudotuberculosis serotype I and Yersinia enterocolitica.
Highlights
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityYersinia pseudotuberculosis, Yersinia enterocolitica
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Yersinia enterocolitica sctE/YopB (Gly246-Val401).
Target T3SS translocon protein SctE, Translocator YopB, Type 3 secretion system translocon protein SctE, Yersinia outer protein B, sctE, yopB
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession P37131
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

Type 3 secretion system translocon protein SctE (YopB) is a ~41 kDa protein. Component of the type III secretion system (T3SS), also called injectisome, which is used to inject bacterial effector proteins into eukaryotic host cells. YopB/SctE and YopD/SctB are inserted into the host membrane where they form a pore and allow the translocation of effector proteins into the cytosol of target cells. Is an essential virulence determinant. Required for YopE translocation.

1. Neyt, C. et al. (1999) Molecular microbiology 33, 971-81. PMID: 10476031
2. Tardy, F. et al. (1999) The EMBO journal 18, 6793-9. PMID: 10581252
3. Lee, VT. et al. (1999) Molecular microbiology 31, 1619-29. PMID: 10209737
4. Nordfelth, R. et al. (2001) Infection and immunity 69, 3516-8. PMID: 11292787
Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Yersinia enterocolitica sctE/YopB Polyclonal Antibody

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