CHO Human CLDN18.2 Stable Cell Line (HX126828)

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カタログ番号	HX126828
説明	CHO Human CLDN18.2 Stable Cell Line (HX126828) is a stable cell line expressing CLDN18.2. Engineered for consistent, high-level target expression in research applications. Highlights ●Stable Expression — Engineered for consistent, high-level target expression.
アクセッション番号	P56856
アプリケーション	FCM
宿主種	CHO
種	Human
ターゲット	CLDN18.2
検出方法	Colorimetric
サンプルタイプ	Plasma, Serum
アッセイタイプ	Quantitative
レンジ	1.56 - 100 ng/mL
感度	0.25 ng/mL
回収率	80-120%
特性	Growth Properties: Adherent; Selection Marker: Puromycin (1 μg/mL)
発送	2-8 ℃
安定性と保存	Frozen in liquid nitrogen.
仕様	1 vial contains at least 2×10^6 cells in 1 mL Freeze Medium
背景	Claudin-18 (CLDN18.2) is a ~27 kDa protein. Involved in alveolar fluid homeostasis via regulation of alveolar epithelial tight junction composition and therefore ion transport and solute permeability, potentially via downstream regulation of the actin cytoskeleton organization and beta-2-adrenergic signaling. Required for lung alveolarization and maintenance of the paracellular alveolar epithelial barrier. Acts to maintain epithelial progenitor cell proliferation and organ size, via regulation of YAP1 localization away from the nucleus and thereby restriction of YAP1 target gene transcription. Acts as a negative regulator of RANKL-induced osteoclast differentiation, potentially via relocation of TJP2/ZO-2 away from the nucleus, subsequently involved in bone resorption in response to calcium deficiency. Mediates the osteoprotective effects of estrogen, potentially via acting downstream of estrogen signaling independently of RANKL signaling pathways. CLDN18.2 is the therapeutic target of zolbetuximab (Vyloy).
注意事項	For research use only. This stable cell line expresses GFP as a reporter marker. GFP is expressed independently of the target protein and is not fused to the target protein.

Flow cytometry

Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CLDN18.2 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

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