HEK293T Human CLDN18.2 Stable Cell Line (HX126818)

Datasheet Share product Copy info

Overview
Images
References
Datasheet

Overview

Catalog No.	HX126818
Description	HEK293T Human CLDN18.2 Stable Cell Line (HX126818) is a stable cell line expressing CLDN18.2. Engineered for consistent, high-level target expression in research applications. Highlights ●Stable Expression — Engineered for consistent, high-level target expression.
Accession	P56856
Applications	FCM
Species	Human
Host species	HEK293T
Target	CLDN18.2
Detection method	Colorimetric
Sample type	Plasma, Serum
Assay type	Quantitative
Range	1.56 - 100 ng/mL
Sensitivity	0.25 ng/mL
Recovery	80-120%
Properties	Growth Properties: Adherent; Selection Marker: Puromycin (1 μg/mL)
Shipping	2-8 ℃
Stability and Storage	Frozen in liquid nitrogen.
Specifications	1 vial contains at least 2×10^6 cells in 1 mL Freeze Medium
Background	Claudin-18 (CLDN18.2) is a ~27 kDa protein. Involved in alveolar fluid homeostasis via regulation of alveolar epithelial tight junction composition and therefore ion transport and solute permeability, potentially via downstream regulation of the actin cytoskeleton organization and beta-2-adrenergic signaling. Required for lung alveolarization and maintenance of the paracellular alveolar epithelial barrier. Acts to maintain epithelial progenitor cell proliferation and organ size, via regulation of YAP1 localization away from the nucleus and thereby restriction of YAP1 target gene transcription. Acts as a negative regulator of RANKL-induced osteoclast differentiation, potentially via relocation of TJP2/ZO-2 away from the nucleus, subsequently involved in bone resorption in response to calcium deficiency. Mediates the osteoprotective effects of estrogen, potentially via acting downstream of estrogen signaling independently of RANKL signaling pathways. CLDN18.2 is the therapeutic target of zolbetuximab (Vyloy).
Note	For research use only. This stable cell line expresses GFP as a reporter marker. GFP is expressed independently of the target protein and is not fused to the target protein.

Images

Flow cytometry

Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CLDN18.2 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet