CHO Human CLDN18.2 Stable Cell Line (HX126828)

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Каталожный номер	HX126828
Описание	CHO Human CLDN18.2 Stable Cell Line (HX126828) is a stable cell line expressing CLDN18.2. Engineered for consistent, high-level target expression in research applications. Highlights ●Stable Expression — Engineered for consistent, high-level target expression.
Номер доступа	P56856
Применения	FCM
Хозяинский вид	CHO
Виды	Human
Цель	CLDN18.2
Метод обнаружения	Colorimetric
Тип образца	Plasma, Serum
Тип анализа	Quantitative
Диапазон	1.56 - 100 ng/mL
Чувствительность	0.25 ng/mL
Восстановление	80-120%
Свойства	Growth Properties: Adherent; Selection Marker: Puromycin (1 μg/mL)
Доставка	2-8 ℃
Устойчивость и хранение	Frozen in liquid nitrogen.
Характеристики	1 vial contains at least 2×10^6 cells in 1 mL Freeze Medium
Фон	Claudin-18 (CLDN18.2) is a ~27 kDa protein. Involved in alveolar fluid homeostasis via regulation of alveolar epithelial tight junction composition and therefore ion transport and solute permeability, potentially via downstream regulation of the actin cytoskeleton organization and beta-2-adrenergic signaling. Required for lung alveolarization and maintenance of the paracellular alveolar epithelial barrier. Acts to maintain epithelial progenitor cell proliferation and organ size, via regulation of YAP1 localization away from the nucleus and thereby restriction of YAP1 target gene transcription. Acts as a negative regulator of RANKL-induced osteoclast differentiation, potentially via relocation of TJP2/ZO-2 away from the nucleus, subsequently involved in bone resorption in response to calcium deficiency. Mediates the osteoprotective effects of estrogen, potentially via acting downstream of estrogen signaling independently of RANKL signaling pathways. CLDN18.2 is the therapeutic target of zolbetuximab (Vyloy).
Примечание	For research use only. This stable cell line expresses GFP as a reporter marker. GFP is expressed independently of the target protein and is not fused to the target protein.

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Flow cytometry

Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CLDN18.2 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

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