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| カタログ番号 | HV376818 |
|---|---|
| 説明 |
HEK293T Human CD344/FZD4 Stable Cell Line (HV376818) is a stable cell line expressing CD344/FZD4. Engineered for consistent, high-level target expression in research applications.
Highlights
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| アクセッション番号 | Q9ULV1 |
| アプリケーション | FCM |
| 宿主種 | HEK293T |
| 種 | Human |
| ターゲット | CD344/FZD4 |
| 特性 | Growth Properties: Adherent; Selection Marker: Puromycin (1 μg/mL) |
| 安定性と保存 | Frozen in liquid nitrogen. |
| 仕様 | 1 vial contains at least 2×10^6 cells in 1 mL Freeze Medium |
| 背景 | Frizzled-4 (CD344/FZD4) is a ~59 kDa protein. Receptor for Wnt proteins. Most frizzled receptors are coupled to the beta-catenin (CTNNB1) canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin (CTNNB1) and activation of Wnt target genes. Plays a critical role in retinal vascularization by acting as a receptor for Wnt proteins and norrin (NDP). In retina, it can be activated by Wnt protein-binding and also by Wnt-independent signaling via binding of norrin (NDP), promoting in both cases beta-catenin (CTNNB1) accumulation and stimulation of LEF/TCF-mediated transcriptional programs. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. 1. Yang, S. et al. (2018) Nature 560, 666-670. PMID: 30135577 |
Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human FZD4 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
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