HEK293T Human LGR5/GPR49 Stable Cell Line (HF614818)

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Número de catálogo	HF614818
Descripción	HEK293T Human LGR5/GPR49 Stable Cell Line (HF614818) is a stable cell line expressing LGR5/GPR49. Engineered for consistent, high-level target expression in research applications. Highlights ●Stable Expression — Engineered for consistent, high-level target expression.
Número de acceso	O75473
Aplicaciones	FCM
Especie huésped	HEK293T
Especies	Human
Objetivo	LGR5/GPR49
Método de detección	Colorimetric
Tipo de muestra	Plasma, Serum
Tipo de ensayo	Quantitative
Rango	1.56 - 100 ng/mL
Sensibilidad	0.25 ng/mL
Recuperación	80-120%
Propiedades	Growth Properties: Adherent; Selection Marker: Puromycin (1 μg/mL)
Envío	2-8 ℃
Estabilidad y almacenamiento	Frozen in liquid nitrogen.
Especificaciones	1 vial contains at least 2×10^6 cells in 1 mL Freeze Medium
Fondo	Leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5/GPR49) is a ~99 kDa protein. Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development.
Nota	For research use only. This stable cell line expresses GFP as a reporter marker. GFP is expressed independently of the target protein and is not fused to the target protein.

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Flow cytometry

Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human LGR5 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

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