HEK293T Human LGR5/GPR49 Stable Cell Line (HF614818)

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Numéro de catalogue	HF614818
Description	HEK293T Human LGR5/GPR49 Stable Cell Line (HF614818) is a stable cell line expressing LGR5/GPR49. Engineered for consistent, high-level target expression in research applications. Highlights ●Stable Expression — Engineered for consistent, high-level target expression.
Numéro d'accès	O75473
Applications	FCM
Espèce hôte	HEK293T
Espèces	Human
Cible	LGR5/GPR49
Méthode de détection	Colorimetric
Type d'échantillon	Plasma, Serum
Type de test	Quantitative
Plage	1.56 - 100 ng/mL
Sensibilité	0.25 ng/mL
Retraitement	80-120%
Propriétés	Growth Properties: Adherent; Selection Marker: Puromycin (1 μg/mL)
Expédition	2-8 ℃
Stabilité et stockage	Frozen in liquid nitrogen.
Spécifications	1 vial contains at least 2×10^6 cells in 1 mL Freeze Medium
Contexte	Leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5/GPR49) is a ~99 kDa protein. Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development.
Note	For research use only. This stable cell line expresses GFP as a reporter marker. GFP is expressed independently of the target protein and is not fused to the target protein.

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Flow cytometry

Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human LGR5 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

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