HEK293T Human LGR5/GPR49 Stable Cell Line (HF614818)

Даташит Поделиться продуктом Копировать информацию

Обзор
Изображения
Ссылки
Даташит

Обзор

Каталожный номер	HF614818
Описание	HEK293T Human LGR5/GPR49 Stable Cell Line (HF614818) is a stable cell line expressing LGR5/GPR49. Engineered for consistent, high-level target expression in research applications. Highlights ●Stable Expression — Engineered for consistent, high-level target expression.
Номер доступа	O75473
Применения	FCM
Хозяинский вид	HEK293T
Виды	Human
Цель	LGR5/GPR49
Метод обнаружения	Colorimetric
Тип образца	Plasma, Serum
Тип анализа	Quantitative
Диапазон	1.56 - 100 ng/mL
Чувствительность	0.25 ng/mL
Восстановление	80-120%
Свойства	Growth Properties: Adherent; Selection Marker: Puromycin (1 μg/mL)
Доставка	2-8 ℃
Устойчивость и хранение	Frozen in liquid nitrogen.
Характеристики	1 vial contains at least 2×10^6 cells in 1 mL Freeze Medium
Фон	Leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5/GPR49) is a ~99 kDa protein. Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development.
Примечание	For research use only. This stable cell line expresses GFP as a reporter marker. GFP is expressed independently of the target protein and is not fused to the target protein.

Изображения

Flow cytometry

Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human LGR5 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

Ссылки

Даташит