HEK293T Human TIGIT Stable Cell Line (HS739818)

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Número de catálogo	HS739818
Descripción	HEK293T Human TIGIT Stable Cell Line (HS739818) is a stable cell line expressing TIGIT. Engineered for consistent, high-level target expression in research applications. Highlights ●Stable Expression — Engineered for consistent, high-level target expression.
Número de acceso	Q495A1
Aplicaciones	FCM
Especie huésped	HEK293T
Especies	Human
Objetivo	TIGIT
Propiedades	Growth Properties: Adherent; Selection Marker: Puromycin (1 μg/mL)
Estabilidad y almacenamiento	Frozen in liquid nitrogen.
Especificaciones	1 vial contains at least 2×10^6 cells in 1 mL Freeze Medium
Fondo	T-cell immunoreceptor with Ig and ITIM domains (TIGIT) is a ~26 kDa protein. Inhibitory receptor that plays a role in the modulation of immune responses. Suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. Upon binding to its ligands PVR/CD155 or NECTIN2/CD112, which are expressed on antigen-presenting cells, sends inhibitory signals to the T-cell or NK cell. Mechanistically, interaction with ligand leads to phosphorylation of the cytoplasmic tail by Src family tyrosine kinases such as FYN or LCK, allowing subsequent binding to adapter GRB2 and SHIP1/INPP5D. In turn, inhibits PI3K and MAPK signaling cascades. 1. Yu, X. et al. (2009) Nature immunology 10, 48-57. PMID: 19011627 2. Liu, S. et al. (2013) Cell death and differentiation 20, 456-64. PMID: 23154388 3. Li, M. et al. (2014) The Journal of biological chemistry 289, 17647-57. PMID: 24817116 4. Reches, A. et al. (2020) Journal for immunotherapy of cancer 8. PMID: 32503945

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Flow cytometry

Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human TIGIT monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

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