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HEK293T SARS-CoV-2 Nucleoprotein (N) Stable Cell Line (VK805818)

HEK293T SARS-CoV-2 Nucleoprotein (N) Stable Cell Line
HEK293T SARS-CoV-2 Nucleoprotein (N) Stable Cell Line
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  • 1 ml
数量:
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概要
カタログ番号VK805818
説明
HEK293T SARS-CoV-2 Nucleoprotein (N) Stable Cell Line (VK805818) is a stable cell line expressing SARS-CoV-2 Nucleoprotein (N). Engineered for consistent, high-level target expression in research applications.
Highlights
  • ●Stable Expression — Engineered for consistent, high-level target expression.
アクセッション番号P0DTC9
アプリケーションFCM
宿主種HEK293T
種Viral (SARS-CoV-2)
ターゲット SARS-CoV-2 Nucleoprotein (N)
特性 Growth Properties: Adherent; Selection Marker: Puromycin (1 μg/mL)
安定性と保存 Frozen in liquid nitrogen.
仕様 1 vial contains at least 2×10^6 cells in 1 mL Freeze Medium
背景

Nucleoprotein (SARS-CoV-2 Nucleoprotein (N)) is a ~45 kDa protein. Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Attenuates the stress granules formation by reducing host G3BP1 access to host mRNAs under stress conditions.

1. Dinesh, DC. et al. (2020) PLoS pathogens 16, e1009100. PMID: 33264373
2. Nabeel-Shah, S. et al. (2022) iScience 25, 103562. PMID: 34901782
3. Dolliver, SM. et al. (2022) PLoS pathogens 18, e1011041. PMID: 36534661
イメージ
  • HEK293T SARS-CoV-2 Nucleoprotein (N) Stable Cell Line

    Western blot

    Untransfected cells and Transfected cells were tested with western blot.

参考文献
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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